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This may be due to the formation of cuprous oxide phase in the oxygen plasma treated films before annealing.
Before annealing only the substrate peak is visible.
Therefore, the RNA-containing oligos are not converted into a cDNA before annealing with complementary DNA.
PA1 and PA2 were coassembled in a 1 9 molar ratio as described above, but redissolved at a final concentration of 15 mg mL−1 before annealing.
Such bonds are common if graphene on copper is exposed to air for long periods before annealing.
C16IDT-BT was spin-coated at 208 rad s−1 (2000 RPM) for 60 s from a 10 mg mL−1 solution in chlorobenzene before annealing at 100 °C for 10 min.
Spectra of the RNA sample were acquired before annealing in the presence of 10 mM potassium phosphate buffer, pH 7.0, and after annealing in the presence of 40 mM of KCl, and 10 mM potassium phosphate buffer, pH 7.0.
(a) Before annealing and (b) after annealing.
The inset image illustrates the multilayer structure before annealing.
This technique showed some advantages before annealing methods.
Insets show optical microscope images of the samples before annealing.
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