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At this stage, the phase volumes were measured and the equilibrium gas phase was collected in a gas pycnometer before analyzing with a gas chromatograph.
Before analyzing with SEM, the surface of algal cells was coated with a gold layer by employing Sputter Coater (Hitachi E-1010, Japan).
Fixed cells were then stained with the cyclin B1 antibody, as described earlier, except that Hoechst was used, and the cells were kept in PBS before analyzing with the HCS system.
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Cells were washed twice in assay buffer before analyzed with flow cytometer.
The cells were filtered through a 70-μm cell strainer to obtain single cell suspension before analyzed with a LSRII flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).
Again, since the DLS measurements demonstrated that the NP-protein complex stabilized at longer time points and did not undergo significant aggregation after centrifugation, the NP-protein complexes were purified by centrifugation followed by a single wash with water before analyzing them with mass spectrometry.
Principal Component Analysis (PCA) was utilized to preprocess the EEG data before analyzing it with the Fast Fourier Transform (FFT) to observe the changes in the main principal frequency bands, delta (0.5 4 Hz), theta (4 7 Hz), alpha (8 12 Hz), beta (13 30 Hz), and gamma (30 40 Hz).
Finally, we will present the related vSDC method and show its results when applied to the DUD-E targets before analyzing its performance with the issue of low-throughput and low-yield experiments.
They were then vacuum-dried and digested with 8 µL trypsin solution before being analyzed with a 4700 Proteomics Analyzer.
Before analyzing evolutionary patterns specifically associated with Sxl recruitment, we tested for global patterns of neutral evolution and positive selection along all branches of the tree (Test 1, see Methods).
While we transiently transfected DLD1 and SW480 cells with β-catenin siRNA and analyzed the gene expression 48 hours after transfection, Mokry et al. [ 99] stably transfected LS174T cells with a doxycycline inducible expression vector encoding β-catenin shRNA and stimulated the cells for 72 hours with doxycycline before analyzing the gene expression profile.
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