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The samples were diluted 1 100 in extraction buffer before analyzing using the ATP determination kit (Invitrogen), according to the manufacturer's instruction.
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Logarithmic transformation was performed on fold change values before being statistically analyzed, using the fold change values of three replicates for each gene measured.
A total of 3570 samples (1972 samples before and 1598 after CHT) were analyzed using the CSS (Veridex, Warren, USA), which consists of the CellTracks AutoPrep System and the CellTracks Analyzer II.
Before and after images were analyzed using the PMOD 3.0 software, and a time-activity curve was generated.
Slope stability before and after excavation is analyzed using the finite element and limit equilibrium analysis methods, respectively.
ALDH scores before and after treatment were analyzed using the two-sided paired t-test.
Before 1970, U-Hg was analyzed using the dithizone method, and afterward using the above-mentioned CVAAS technique expressed in micrograms per liter.
The differences within the treatment group before and after the intervention were analyzed using the t-test statistic, with p-values calculated using 5000 permutations.
In order to define a starting condition, the test plates were analyzed using the BonNDTinspect system before every contamination procedure.
Accordingly the supplementation strategy for adding HDF to NHKs was next analyzed using the computational model before it was applied to real cell cultures to see to what extent HDFs might differ to irradiated HDFs in supporting keratinocyte colony expansion.
Although the authors of the original studies reportedly examined the quality of their hybridizations before analysis, all genomic data were re-analyzed using the ChipDX Quality Module, which was specifically designed for diagnostic applications.
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