Sentence examples for before analyzing on a from inspiring English sources
The DNA cell samples from fixed lysed whole blood of Tasmanian devil and human lymphoblastoid cell lines were stained with PI solution before analyzing on a flow cytometer (Darzynkiewicz and Juan, 1997 ).
Fixed cells were permeabilized, digested with DNAase I, labeled with Alexa647-conjugated anti-BrdU Ab, washed and resuspended in the presence of 7-AAD before analyzing on a flow cytometer.
Freshly prepared Calpain-Glo™ Reagent was mixed with samples (1 1) and added to each well of a white 96-well plate containing 40 μl of blank or test sample and incubated at room temperature for 15 min before analyzing on a LumiCount luminometer.
We sequenced the amplified regions in the Col-0 and Ws-2 parents and the F2 individuals with the predicted CO in sequencing by assembling reactions containing 1 µM primer, 0.5 µL of BigDye Terminator ready reaction mix, and 1X sequencing buffer and incubating for 20s at 96°, followed by 29 cycles of 10 sec at 50° and 4 min at 60° before analyzing on an ABI 3730xL sequencer.
Briefly, the cells (1 × 10 cells) were suspended in 100 μl binding buffer, and then treated with 5 μl of Annexin V-PE (BD Pharmingen) and 5 μl 7-AAD before analyzed on FACSCanto II flow cytometer and BD FACSDiva software v5.0.3 (Becton Dickinson, Franklin, NJ, USA).
Cells were fixed, membranes permeabilized and samples were then treated with propidium iodide, before being analyzed on a FACS Calibur (Becton Dickinson).
After two washes in PBS 1×, cells were then fixed in PFA 1% before being analyzed on a Galaxy flow cytometer (Partec, Germany) with FlowMax software (Partec, Germany).
DNA that remained bound to the beads was extracted by phenol/chloroform, precipitated and resuspended in TE before being analyzed on a 1% agarose gel or a 15% polyacrylamide gel stained by Ethidium Bromide.
The cell pellet was stained with propidium iodide (10 µg/ml) (Sigma) in PBS (Flowlab) containing RNase A (100 µg/ml) and was incubated in a 37°C water bath (Memmert) for 30 minutes in the dark before being analyzed on a FASCalibur flow cytometer (Becton Dickinson, USA).
The gels were dried before being analyzed on a Phosphorimager.
The pellets were resuspended very gently with wide-bore pipette tips just before being analyzed on a FACSCalibur flow cytometer (Becton Dickinson).
