Sentence examples for before analyzed using a from inspiring English sources
Finally, samples were stained with propidium iodide (Sigma-Aldrich, St .Louis, Missouri, USA) and incubated on ice for 30 minutes before analyzed using a BD FACSCaliburTM flow cytometer (BD Bioscience, Franklin Lakes, NJ, USA).
The cell suspensions were combined with 5 μl of annexin V-FITC and 5 μl of propidium iodide and incubated at room temperature for 5 min in the dark, before being analyzed using a BD FACSCanto II flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA) using Mod Fit LT™ 3.3 software.
The mixtures were boiled in a water bath for 15 min and left at room temperature for 10 min before they were analyzed using a UV/VIS Spectra Lambda 35 (PerkinElmer).
While Tang et al. (2010) used a "multiple region brute-force" fiber tracking method before FA maps were analyzed using a region of interest approach, we analyzed whole-brain DTI scans without a priori hypotheses using TBSS calculating maps of FA, RD, and AD.
The data were analyzed using a gradual before-after control-impact (gBACI) model.
To exclude dead cells, 5 ng propidium iodide (PI; Sigma) was added just before analysis and cells were analyzed using a FACSCalibur cytometer and CellQuestPro software.
Data from the GOHAI questionnaire before and after implant treatment were analyzed using a General Linear Model procedure (GLM) (variable: GOHAI parameters; fixed variable: type of oral treatment).
The RNA was analyzed using a Bioanalyzer before being labeled with Cy 3 and Cy 5 for the normoxic versus hypoxic samples, respectively.
Symptoms outcome before and after surgery were compared and analyzed using a five-point-ranking scale.
The hazard and amenity effects of the WNP before and after a landslide event are analyzed using a difference-in-difference approach with a random coefficient model.
Following incubation, the samples were centrifuged before H2 content of the supernatant was analyzed using a membrane inlet mass spectrophotometer (Bay Instruments, Easton, MD, USA) which has been shown effective in measuring dissolved gases in water [ 74] and cell culture samples [ 75].
