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Before analysis with the FT-IR apparatus, the standard glasses were mounted in an epoxy resin, and then singly polished with a 1-μm grain diamond paste.
The 400-m run time data were skewed and successfully normalized before analysis with the reciprocal function (i.e., transformed value = 1/original value).
All samples were normalized before analysis with the GCOS 1.4 software (Affymetrix), (i.e. conservation of the 75th percentile of each probecell).
The categorisation of adjustment variables was prespecified before analysis, with the exception of birth weight.
SAA was stable both at room temperature and refrigerated, and thus samples may be stored before analysis with the reference method.
Stool specimens were collected and immediately stored at –20°C before analysis with the H. pylori stool antigen test (Meridian Bioscience Inc., Cincinnati, OH, USA).
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For inhibition experiments, cells were incubated for 30 min before analysis with one of the following inhibitors: 100 μM 2-APB for blocking IP3Rs, 0.5 μM TG for SERCA ER Ca2+ pump inactivation.
Where appropriate, we categorized responses before analysis, with categories selected on the basis of the distribution of responses for that variable.
We thus spiked each population sample before analysis with 914 individuals from the HGDP-CEPH panel [6] originating from several geographic locations in Africa, Europe and Asia (see Materials and Methods).
Before analysis with HPLC-ICP-MS, the samples were thawed and dissolved with ammonium carbonate (20 mM, pH 9.0) to minimize any sulfur oxygen exchange while awaiting analysis (Conklin et al. 2006).
The embryos were then cultured to the mid-blastocyst stage before analysis with confocal microscopy.
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