Sentence examples for before analysis using a from inspiring English sources

Cells were then washed three times with PBS, before analysis using a Becton Dickinson FACS Calibur flow cytometer.

Erythrocytes in blood samples were lysed before analysis using a FACS lysing solution (BD Biosciences) according to the manufactures instructions.

The membrane was washed twice at high stringency (0.1× SSC, 0.1%SDS) at 60°C before analysis using a Typhoon Phosphorimager (Amersham Biosciences) and the ImageQuant software.

To mount adult wings, flies were pre-incubated in 80% ethanol and 20% glycerol solution, then dissected and mounted in 70% glycerol before analysis using a Leica light microscope.

Finally, Propidium Iodide (PI, 1 µg/mL) was added to the cells before analysis using a FACS Calibur Flow Cytometer BDD) with at least 100,000 events for each measurement.

All the fixed cells were incubated in PBS with 5 µg/mL propidium iodide, 0.1 mg/mL RNase A and 0.5% Triton X-100 for 45 min at 37°C before analysis using a Becton Dickinson FACS analyzer (Oxford, UK).

The sample was prepared by resuspending the extracts in 5 mL of double-distilled water before analysis using an Elan 6100 DRC ICP/MS (Perkin-Elmer).

Cellular proliferation was detected by 3H-thymidine incorporation into replicating DNA was measured by adding 1 µCi to each replicate well of 105 PBMC for 24 hours before quantitative analysis using a Topcount® Liquid Scintillation Counter (Perkin-Elmer, Waltham, MA).

A calibration curve was constructed before each analysis using a series of standard solutions of 5,6-dihydro-M1dG and 5,6-dihydro-[C3]M1dG 5,6-dihydro-[C3]M1dG 5,6-dihydro-[C3]M1dG

A calibration curve was constructed for each diastereomer before each analysis using a series of standard solutions of CpopC and [N3]CpopC.

Wells were then washed three times with distilled water and air dried before microscopic analysis using a BH2 microscope (Olympus Optical, London, UK) linked to a KY-F55BE video camera (JVC, London, UK).