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Samples were washed before analysis to remove any external contamination.
All samples were filtered through a 0.2 μm filter before analysis to remove particles.
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Therefore, the cells were trypsinized before the flow cytometry analysis to remove any protein bound to cell membranes.
Following day 0, any data that did not cover an entire 24 hour day (0800 to 0800) for a calf were removed before analysis to ensure the entire calf activity was recorded for all 24 hours due to calf activity varying throughout the day [ 26].
Because written informed consent was not obtained, any identifying information was removed from the samples before analysis to ensure strict privacy protection (unlinkable anonymization).
Identifying details from the optional Section 5 of the questionnaire were removed from each response before analysis, to anonymise the data.
The raw spectral data were preprocessed by WiRE 2.0 software (Renishaw, Great Britain) before the Data statistical analysis in order to remove the interference noises and oversaturated spectra.
Before analysis, we pruned the sequences to remove fragments and redundant sequences [4].
The supernatant was removed with care and filtered through cellulose filters (0.22 μm) in order to remove before analysis any possible agglomerated nanoparticulate matter.
At a defined time interval, about 3 mL of solution was extracted from the reactors and then centrifuged to remove catalysts before analysis.
All samples were filtered through a 0.2 μm filter to remove particles before analysis.
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