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PI was then added at 0.2 μg/mL right before analysis to exclude dead cells.
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The first and terminal steps of a row of data of each trial were removed before data analysis to exclude the effects of acceleration and deceleration.
A multicollinearity test was carried out before the regression analysis in order to exclude confounded variables because of a significant correlation of moderate strength (r > 0.4).
Before starting the statistical analysis, we decided to exclude very low expression tags from the study.
PI at a final concentration of 10 μg/ml was added to each sample immediately before the measurements to exclude non-viable cells from the analysis.
DAPI (4,6 diamidino-2-phenylindole) (Molecular Probes, now part of Invitrogen Corporation, Carlsbad, CA, USA) was added immediately before cytometric analysis to determine or exclude dead cells.
In this study, to maximize the quality of LCLs, each aliquot of LCLs was maintained in culture for no longer than 3 months (less than 160 'population doubling levels') and cells were karyotyped before microarray analysis and before each experimental validation to exclude the possibility of chromosomal rearrangements (other than full trisomy 21).
Those who filed a tax return before landing (i.e., who lived in Canada before becoming permanent residents) and those who arrived before the age of 25 are excluded from the analysis as a way to exclude immigrants who might have acquired education in Canada before becoming permanent residents.
7-amino-actinomycin d (7-ADD, Sigma) was added to the cells to exclude dead cells before analysis.
Immediately before flow cytometry analysis, PI (1 μg/ml) was added to exclude the non-viable cells.
Before analysis, we excluded responses from three participants who had outlier WTP per QALY values since they might lead to biased results.
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