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DAPI was then added before analysis on a BD LSR-II.
Cells were then washed in PBS and just before analysis on a FACSCanto TM (Becton Dickinson).
Cells were incubated with FACS antibodies for 15 minutes at 4°C and washed once with PBS before analysis on a FACS Calibur BD Biosciencess).
The cells were incubated with 5 µl APC-conjugated Annexin V antibody (BD Biosciences) and 5 µg/ml PI at room temperature in the dark for 15 minutes before analysis on a FACSCalibur cytometer (BD Biosciences).
Cell suspensions were vortexed briefly before analysis on a BD FACSCalibur flow cytometer.
Before Analysis on a FACSAria flow cytometer (BD Biosciences), the cells were diluted in 300 μl BSA/PBS.
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Cultures were diluted to a final optical density of 0.01 before analysis on an LSRII flow cytometer (BD, Franklin Lakes, NJ).
Digests were separated by electrophoresis on a 1.5% agarose gel and stained with ethidium bromide before analysis on an AlphaImager3400 (Alpha Innotech).
Products from sequencing reactions were purified on Cetri-Sep spin columns (Princeton Separations, Adelphia, NJ, USA) before analysis on an ABI 3130xl Genetic Analyzer.
For confocal microscopy, meshes were mounted between glass slides and coverslips with ProLong anti-fade reagent (Invitrogen) before analysis on an LSM-510 confocal microscope (Zeiss) using a 20× air lens (Zeiss Plan-APOCHROME, NA 0.8).
All of the samples were filtered through a 0.20-μm filter and diluted with an eluent before analysis on HPLC.
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