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Cells were then washed in PBS and just before analysis on a FACSCanto TM (Becton Dickinson).
Cells were incubated with FACS antibodies for 15 minutes at 4°C and washed once with PBS before analysis on a FACS Calibur BD Biosciencess).
The cells were incubated with 5 µl APC-conjugated Annexin V antibody (BD Biosciences) and 5 µg/ml PI at room temperature in the dark for 15 minutes before analysis on a FACSCalibur cytometer (BD Biosciences).
The reactions were performed at 30°C for 30 min before analysis on SDS-PAGE.
An injection standard was added before analysis on GC/MS (ECNI).
Samples were filtered through a 0.20-μm filter and diluted with eluent before analysis on HPLC.
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We discuss advances in the core concurrency analysis techniques for event-driven applications with focus on happens-before analysis, race detection, and model checking.
For example, it is possible that fusion in the crypt epithelium represents an initial fusion event in a proliferative cell that occurred only a short time before analysis (perhaps on day 5 6).
Cell debris was gated out before analysis based on light-scattering properties.
All continuous confounders were categorized into quartiles before analysis based on their distribution in the combined group of cases and controls.
Therefore, before analysis, scores on the energy and macromolecules and CO2 and ocean acidification assessments were adjusted to normalize scores to scientific design.
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