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Cell aggregates were allowed to differentiate for a further two days before analysis for the presence of GFP positive cells (Figure 4).
The samples were pooled by herd, before analysis for the presence of C. burnetii DNA.
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Therefore, it is essential that the bottles were vigorously shaken before analysis for quantitative recoveries.
In the laboratory, samples were routinely centrifuged before analysis for 5 min at 10,000 revolutions per minute to obtain serum.
Data were log transformed as appropriate before analysis for normalisation purposes.
Probe intensities are log2 transformed before analysis for both gene expression and methylation data.
Serum was separated and frozen at −80 °C before analysis for FA content by a single technician.
A logarithmic transformation using base 2 (Log2) was applied before analysis for PC20 (doubling doses scale).
As mentioned before, analysis for TS14 should be considered in PWS patients without chromosome 15 disturbances.
Total nucleic acid was extracted from plasma pools as described previously (4 ) before analysis for PARV4 DNA.
Consequently, for each animal, the bone marrow of both tibias was flushed with 200 μl of DMEM and mixed before analysis for Dkk-1 levels.
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