Sentence examples for before analysing using a from inspiring English sources

After 30 sec, 600 µl acridine orange (AO; Sigma) diluted in AO staining solution (37 mM citric acid, 126 mM Na2HPO4, 1 mM EDTA, 0.15 M NaCl, pH 7.4 with AO added fresh to a final concentration of 6 µg/ml) was added to the sperm, mixed and incubated for 3 min at room temperature before analysing using a fluorescent assisted cell sorting (FACS) machine.

Cells were subsequently incubated in the dark (22°C, 30 mins) before analysing using a Tali Image-Based Cytometer (Invitrogen, USA).

Following two PBS washes, cells were fixed in 0.1% formaldehyde overnight before being analysed using a FACScalibur flow cytometer (Beckton Dickinson).

The cells were then incubated with Alexa Fluor 488 donkey anti-goat secondary antibody (1/800 dilution) for 30 min at room temperature before being analysed using a flow cytometer.

The cotton swab was then centrifuged (Hettich Centrifuge EBA 20, Andreas Hettich Group, Ltd., Tuttlingen, Germany) at 2400 x g at room temperature for 2 minutes and the saliva extracted was stored at −20°C before being analysed using a commercially available cortisol ELISA kit (Cortisol ELISA, IBL International, Hamburg, Germany) validated for pig saliva [ 18].

The scores given for knowledge before and knowledge after were analysed using a Mann-Whitney test which was significant (p value of <0.001), indicating that students felt their knowledge had improved after the session.

For all postal codes, distances between partners before shared living are calculated and analysed using a Geographic Information System.

Differences between assessments (before treatment and after surgery) were analysed using a paired t-test.

Sera obtained immediately before the first infusion of trastuzumab were analysed using a Sequential Solid Phase Sandwich Human Her-2/ neu Quantitative ELISA (Her-2/ neu Microtiter ELISA, Oncogene Science, Cambridge, MA, USA) according to the manufacturer's instructions.

These data were analysed as before using a repeated-measure ANOVA having within-participant factors of Source (images, sentences or pictures) and Valence (negative or benign).

In this case, single-locus approaches are a necessary step before further analyses using the multi-locus or candidate gene approaches.