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The containers were weighed before analysing the sample, to weigh the sputum and determine the sample validity.
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Just before analysing the samples they were diluted a further 1000× with Milli-Q water.
Verbal informed consent was obtained from all the participants before analysing the stool sample.
Thereafter, the crushing chamber is baked at about 150 200 °C for 72 h before loading and analysing the samples at room temperature the next day.
The recovery of the method was determined by analysing the samples before and after the addition of the pure MCYST and then subtracting the concentration of the MCYSTs present in the sample prior to spiking.
OD values of blank samples were subtracted from sample OD values at the respective time points before analysing the data.
Before analyses, the samples were centrifuged and filtered with a 0.2 µm filter.
The porosity of the materials was examined by N2 adsorption at 77 K (AUTOSORB 1, Quantachrome); before analyses the samples were outgassed overnight at 80 °C.
Before analyses, the samples were centrifuged for 15 min at 3000 rpm, and diluted 1 2 in a sample matrix.
Dr Stephen Morley, who analysed the samples, said there would be "significant effects".
Before analyses, the blood samples had been stored for up to 13 years at −80°C.
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