In the comparison study samples were analysed on the in-clinic assay on the day of collection and then stored up to 5 days at 4°C before analyse on the reference method.
In the regression analyses presented in Tables 4 and 7, confounders were specified before the analyses on the basis of unpublished data (work, ethnicity, residence and educational level (manuscript submitted)).
The cells were washed and re-suspended in FACs buffer containing 100 ng/ml DAPI before analysing on an LSRII flow cytometer.
SEC analyses on the samples before and after azidation revealed no changes in the molar mass or the dispersity, proving that the modification had no deleterious effect on the polymer samples.
In 61 patients, the levels of the cytokines IL-1ß, sIL-2R, IL-6, IL-8, IL-10 and TNF-α were measured as a part of the routine blood analyses on the morning before start of conditioning, twice during the conditioning period and after HSCT, as well as two times per week up to the time of clinical discharge.
Additionally, we conducted analyses on the trials immediately before and after the downshift.
0·2 × 10 cells were fixed overnight in 70% ethanol, washed twice in PBS and resuspended in 300 μl PBS with 50 μg/ml PI and 200 μg/ml RNAse A. Cells were incubated at 37°C for 30 min before being analysed on the flow cytometer.
A total of 0.2 × 10 cells were fixed overnight in 70% ethanol, washed twice in PBS and resuspended in 300 µl PBS with 10 µg/ml DAPI and 200 µg/ml RNase A. Cells were incubated at 37°C for 30 min before being analysed on the flow cytometer.
Briefly, 0·2 × 10 cells were washed twice in PBS and resuspended in 300 μl PBS with 10 μg/ml Hoescht 33342 and 0·5 μg/ml Pyronin Y. Cells were incubated at 37°C for 45 min before being analysed on the flow cytometer.
Before analyzing the prediction models, we started with some general analyses on the EQ-5D.
