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This increase in ultimate strength is accompanied by a decrease in the amount of elongation that the fibre can sustain before reaching its breaking point; the molecules are not able to slide over one another as they could before alignment took place.
Surface modification of SiNWs can be done before alignment.
Before alignment, ITS1 and 28S segments were cut from the sequences where needed.
Erroneous sequence tags were eliminated using quality values before alignment.
Before alignment, 5% off the end of any sequence added from the gene model extension, upstream or downstream, was trimmed.
There is no need to remove any partial adaptor sequence from the sequence reads as the algorithm automatically trims this before alignment.
Since both short read clusters and candidate splicing sites were predicted before alignment, there was no need to construct the junction library.
Before alignment, primer sequences were removed from both ends of the sequences, so that all sequences begin and end at homologous sites.
Additionally, for pair-end reads alignment, MAW can be used to quickly check the splicing junctions before alignment, which could potentially improve the efficiency of splicing event identification.
In this study, a total of 36,864 HinDIII and EcoR1 clones (average size 128 kb and 124 kb respectively) formed an initial build of 208 contigs containing 30,195 BAC clones representing 303 Mb before alignment with genome sequence contigs.
No assembly was performed before alignment.
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