Sentence examples for before addition of samples from inspiring English sources

Plates were blocked with 5% (w/v) non-fat dried skimmed milk powder in PBS before addition of samples or standards diluted in PBS with 0.05% (v/v) Tween 20 and 1% (w/v) BSA (Sigma, #A7030).

Before addition of sample into respiration chambers, a baseline respiration rate was determined.

Beads were incubated with lysates overnight at 4 °C and washed three times in lysis buffer before addition of sample buffer (100 mM Tris, pH 6.8, 200 mM DTT, 4% SDS, 0.2% bromophenol blue and 20% glycerol) followed by boiling for 2 min.

For the Cel7A and Cel6A ELISA's, before the addition of samples to the ELISA plate, the purified enzyme samples or the hydrolysate samples were first heated at 100°C for 10 minutes.

The induced cells were then chilled on ice for 15-30 min before addition of sterile aluminum samples for 15 min at 37 °C with shaking.

For chromatin fractions, the nuclear extracts were treated with "no salt buffer" (3 mM EDTA and 0.2 mM EGTA) before addition of 2× Laemmli sample buffer.

0.5 μl of the matrix solution was added to a Bruker™ Polished Steel 384-well plate and allowed to dry completely before addition of 0.5 μl sample on top of the dried HPA droplet.

Third, before addition of proteinase K, sample and control DNA were incubated with RNase A (50 μg/ml) for at least 90 min at 42°C (except in the σ-analysis shown in Fig. 8A where incubation was 30 min as indicated).

In the gel, 4 mm wells were punched before addition of 20 μl of sample in each well.

Pellets were resuspended in hypotonic buffer for 10 min before the addition of sample buffer, whilst sample buffer was added directly to all supernatant fractions.

Samples were incubated at 30 °C for 40 min before addition of 4 × SDS sample buffer and boiled for 5 min.