Sentence examples for before addition of each from inspiring English sources

Slides were given two 5 min washes in TBS at pH 7.6 before addition of each reagent.

The commercial feed was grounded into powder with a laboratory grinding machine and sieved before addition of the feed additives.

In the gel, 4 mm wells were punched before addition of 20 μl of sample in each well.

To determine MBC, an aliquot of the inoculum was removed from each well before addition of resazurin and plated onto tryptic soy agar.

ABTS and 0.1 M citrate phosphate buffer were incubated at the optimum temperature of each protein 2 min before addition of enzyme.

Plates were washed in between each step and before addition of Ultra TMB-substrate and 0.1 M H2SO4 as described above.

Solutions 1 and 2 (25 μL of each) were combined for approximately 1 min before addition of 50 μL of solution 3 to initiate the reaction.

Cells were cultured for 1, 2, 3, 5, and 7 days before addition of 10 μl CCK-8 to the culture medium in each well.

The enzyme was incubated with each reagent for 10 min at 50°C before addition of p-nitrophenyl-β-D-glucopyranoside to initiate the enzyme reaction.

Each sample was placed into an individual tube before addition of 1 mL of TRIzol reagent (Invitrogen), followed by homogenization with a Polytron mixer (Kinematica).

The next day slides were washed 5× for 5 minutes each in PBS + 0.2% Triton X-100 before addition of secondary antibody.