Sentence examples for before addition of dsRNA from inspiring English sources

Before addition of dsRNA to the cell culture media cells were pre-incubated for 1 hour in serum free medium.

Cells were assayed 4 days following addition of dsRNA.

Mock-treated and mock-depleted cells were transfected without the addition of dsRNA or with Rho1 dsRNA, respectively.

Addition of dsRNA targeting either key or akirin to the culture medium strongly reduced attacin-A-luciferase expression, compared to control (GFP) dsRNA knockdown (Fig 2B).

Our study demonstrates that transfection of dsRNA results in the cleavage of apoptotic marker Caspase-3 and PARP in MSCs, whereas addition of dsRNA only slightly induces apoptotic response till reaching a concentration as high as 25  μg/ml.

Detection of the initial phenotype resulting from addition of dsRNA to the cell culture is an important goal of the RNAi experiment.

20 μg of dsRNA was added to cells and they were incubated at 25°C for 1.5 hours before addition of 3 ml Schneider's media containing 10% FBS and 0.1 mg/ml gentamicin.

Lidocaine ( 10−6to 10−4m) was applied 15 min before addition of phenylephrine.

before addition of COOH-MWCNTs (COOH-MWCNT+).

overnight before addition of protein beads.

After addition of naked dsRNA or bacteria to the S2 cell cultures RNase III (2 units) was added to the cell culture media.