Sentence examples for before adding the mixture from inspiring English sources

For antibody cross-linking, Fc-specific goat-anti-human IgG antibody or F ab' 2 fragment (Sigma-Aldrich) was incubated with one-armed YW211.31 or Apomab antibody for 1 h before adding the mixture to cells.

In the in vitro studies, cells were transduced with LVVs by diluting the vector into prewarmed media before adding the mixture to cells.

Then we mixed the S1P standard and samples with the anti-S1P antibody before adding the mixture to the S1P-coated plate.

To specifically evaluate the role of BDNF in OCM, a neutralizing anti-BDNF antibody (Promega) was pre-incubated for 2 h (at 4°C) with OCM before adding the mixture to neuronal cultures.

As previously mentioned, the mixture was left for 24 hours in order to allow a fixed amount of CisPt (8  μg/mL), together with different molar concentrations of carnosine (1 : 1.51.5 : 1, 2 : 1, and 2.5 : 1), to complex before adding the mixture to the HepG2 cells.

Add butter and a little bit of flour into the baking pan before adding the mixture.

The virus/compound mixtures were then incubated for 16 hr with HFFs (1.7 × 10/well) in a 96-well plate that was seeded 1 day prior to infection and washed twice with PBS before adding the mixtures.

The other, for people who like over-the-top intensity, is to spread a thin layer of melted semisweet or bittersweet chocolate on the baked crust before adding the walnut-caramel mixture.

Let the mixture cool down before adding the yeast mixture to it.

The reaction system was cooled naturally to reach room temperature before adding the DOX mixture.

If you are willing to prepare your jelly mould before adding the jelly mixture to set, you can use an alternative method.