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This was repeated once before acidification of samples with 200 μL of 6 M HCl.
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Active TGF-β1 was measured before and after acidification of samples (to convert latent TGF-β1 to active TGF-β1) using the ELISA and/or MLEC assays.
Additionally, the sample powders were analysed for total carbon (TC) and total organic carbon contents (TOC, after acidification of samples to remove carbonate) using a Leco CS-300 analyser.
In some cases the virus was allowed to bind to the target liposomes at neutral pH before acidification of the medium, and in other assays virus and liposomes were added directly to buffer at pH 5.0.
Although water samples from NG1 typically were processed and analyzed within 12 h (without adding HCl), the acidification of the samples from other sites with HCl probably led to the overestimation of the dissolved reactive nutrients, especially SRP.
Considering the absence of strongly acidic rosin-sized papers, the hypothesis that bi-modality is a result of acidification of some samples during degradation could be put forward.
In order to determine the total level of cadmium in a serum sample, cadmium must be extracted from these proteins, a process most effectively achieved by acidification of the sample.
As mentioned before the acidification of either drinking water or feed has been found to reduce pathogen transmission before in different studies.
Following acidification of the wine sample lead ions can be reduced at the Hg/Pt electrode surface at −1.0 V (vs SCE).
Each sample is determined not just by the sample before it, but the thousands of samples that came before it.
0.1 g of the xylenol orange solution were prepared by dissolving 0.1 g of xylenol orange solution in water, before acidification with 2 3 drops of hydrochloric acid (1 4).
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