Sentence examples for before a final wash from inspiring English sources

Finally, the cells were incubated for 5 min in IB prewarmed to 37°C before a final wash into ice cold IB.

protected from light, the slides were repeatedly washed with PBS, then incubated in 10 mM acetate buffer, pH 6, containing 1 mM CuSO4 for 10 min before a final wash in PBS.

Non specific stain was removed with 3% acetic acid and rinsed in water for 10 min. The slides were then oxidized in 1% periodic acid in water at room temperature for 10 min, washed in water for 5 min, immersed in Schiff's reagent for 10 min, rinsed in water for 5 min and then three times in 0.5% sodium meta-bisulphite before a final wash in water.

were then added for a secondary hybridization at 65°C for 1 hour before a final wash (15 ).

The coverslides were then washed 3 times at 45°C with 50% formamide in SSC2X, one time in SSC2X before a final wash in SSC2X, 0.1%NP-40NP-40

Samples were washed and resuspended in cold flow cytometry staining buffer (1% BSA/PBS); stained for 30 min in the dark before a final wash and acquisition.

FITC-conjugated streptavidin was added (1 50 in 1% BSA/PBS, Vector Laboratories) for 30 min at 4 °C before samples were given a final wash.

The cells were then washed in 1% BSA, with a final wash in PBS, before mounting on coverslips with mounting media (Dako Inc .. Cells were imaged on a Zeiss 510 confocal microscope using an oil immersion 63X objective with a NA of 1.4.

Staining for the proliferation marker Ki67 (MIB1 antibody) was performed on cells fixed in 3.7% formaldehyde/0.15 M NaCl for ten minutes, five minutes in 100% ethanol with a final wash in PBS before assay.

A final wash step was performed before incubation with NiDAB SK-41000 substrate kit; Vector Labs) for visualisation.

After six washes with PBS containing 0.1% (w/v) dodecyl-maltoside (final concentration), a final wash with PBS was used before eluting the immunoprecipitates with 2× SDS- Laemmli sample buffer.