Sentence examples for before a final extension from inspiring English sources

This was repeated for 30 cycles before a final extension at 72 °C for 10 minutes.

The mixture was cycled six times at 52°C for 10', 50°C for 15', and then gradually cooled from 50°C to 37°C over two minutes before a final extension for 90" at 37°C.

PCR cycling conditions were as follows: 95°C for 5 min; 35 cycles of 95°C for 30 s, 55°C for 45 s, 72°C for 30 s before a final extension at 72°C for 4 min.

The cycling conditions for each PCR were an initial denaturing step of 15 min at 95°C, then 40 cycles of 95°C for 50 s, 50°C (60°C for gp60) for 50 s, and 72°C for 60 s before a final extension of 10 min at 72°C.

The reaction conditions were 94°C (2 min) for one cycle, and then 94°C (30 s) and 55°C (30 s) and 72°C (30 s), for 24 - 32 cycles, before a final extension of 72 C for 10 minutes.

PCR conditions were as follows: one denaturing cycle at 95°C for 5 min, followed by 30 cycles of 94°C for 30 s, 60°C for 30 s, and 72°C for 1 min before a final extension step at 72°C for 10 min. These primers resulted in a primary PCR product of 496 bp, which was sequenced to assure a 100% nucleotide match with BNIP3 mRNA.

PCR procedure consisted of 35 cycles of denaturation at 94°C for 15 sec, annealing at 60°C for 60 sec, with an initial denaturation of the DNA at 94°C for 5 min before PCR, and a final extension at 72°C for 5 minutes.

A year later, a final extension completed the route to Owen Sound.

The PCR conditions were similar to those applied before, with the exception of a final extension of 30 min in this case.

The amplification reaction: 94°C for 3 min, followed by 35 cycles at 94°C for 30 s, 60°C for 30 s, and 72°C for 30 s, and a final extension at 72°C for 5 min before cooling to 4°C.

Thermocycling began at 95°C for 10 min, followed by 35 cycles of 94°C for 1 min, 55°C for 1 min, 72°C for 1.5 min and a final extension at 72°C for 10 min before holding at 12°C.