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Briefly, trophocytes and fat cells from adult worker bees were fixed with 4% paraformaldehyde in PBS for 20 min, washed with PBS three times, permeabilized with 0.2% Triton X-100 in PBS for 2 min, washed with PBS three times, and incubated with 1% bovine serum albumin (BSA) for 1 h.
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In the PER group, bees were fixed in the same holders used for SER conditioning and they experienced one odorant associated with sucrose solution during 6 trials and another non-rewarded odorant during 6 trials.
After chilling to immobility, bees were fixed to a custom made imaging stage using dental wax (Kerr; Siladent).
Cells were fixed with 4% paraformaldehyde.
The cultures were fixed with 4% paraformaldehyde.
Ileum sections were fixed with 4% paraformaldehyde.
Cells were fixed with 4.2% formaldehyde.
Plants were fixed with medical adhesive (Hollister).
During the experiments, specimens were fixed on their elytra with paper glue or bee wax.
Hackenberg's bees were filled with black scar tissue.
Different groups of bees were fed with different substances.
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