Sentence examples for bees were fixed to from inspiring English sources
After chilling to immobility, bees were fixed to a custom made imaging stage using dental wax (Kerr; Siladent).
To help bees navigate accurately, four geometric patterned posters (height = 120 cm; width = 85 cm) were fixed to the walls in each corner of the room as landmarks (Fig. 2, for details see Fig. S2).
In the PER group, bees were fixed in the same holders used for SER conditioning and they experienced one odorant associated with sucrose solution during 6 trials and another non-rewarded odorant during 6 trials.
Briefly, trophocytes and fat cells from adult worker bees were fixed with 4% paraformaldehyde in PBS for 20 min, washed with PBS three times, permeabilized with 0.2% Triton X-100 in PBS for 2 min, washed with PBS three times, and incubated with 1% bovine serum albumin (BSA) for 1 h.
The number of employed bees and onlooker bees were set to 10.
Briefly, the purified IGs, purified IDVs, or trophocytes from worker bees on the second days after eclosion were fixed in 2.5% glutaraldehyde, postfixed in 1% osmium tetroxide, dehydrated through an ethanol series, and embedded in Spurr's resin.
During the experiments, specimens were fixed on their elytra with paper glue or bee wax.
