The combined efforts of academia and industry were able to overcome numerous substantial challenges: the target was not yet validated in vivo, the viral lifecycle was poorly understood, no robust preclinical models in cell culture or animals were available, and no structure of the viral protease had been solved.
Although the WHO-5 was not yet validated in Bangladesh, it was found reliable and effective among elderly Indian communities [ 21], which are socio-economically similar to Bangladeshi communities.
A biological interpretation of miRNA function in MS is complicated by the fact that most of the miRNA targets are predicted from bioinformatics analysis and are not yet validated in biological studies.
Second, the questionnaire we used was based on the SLENQ, but was not yet validated in Dutch.
This approach overcomes most of the drawbacks of original FSE sequences and seems to be effective for the identification of areas of regional hyperemia, although it is not yet validated in patients with EMB-proven AM.
The KIDSCREEN-52 is not yet validated in children aged 6 and 7.
Definite thresholds of cytotoxic and vasogenic oedema established by ADC are not yet validated in a wide population of patients or in animal studies.
It is important to mention that during the protocol development of the oral health study in the Pelotas cohort, the Child-OIDP version [ 27] was not yet validated in Brazilian Portuguese.
Given the population of Iran (over 70 million) and high prevalence of OA of the knee in the country, the AIMS2, as an internationally recognized instrument for patients with osteoarthritis, was not yet validated in Iran.
Since the AGE reader is not yet validated in populations with a darker skin type we restricted our analysis to Caucasians with a skin reflectance of at least 6%.
