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For colorectal carcinoma, such changes have been well enumerated by Vogelstein and coworkers [ 8, 9].
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While such designs are well suited to enumerating the beliefs that influence behavior, they are less amenable to understanding how individuals perceive, conceptualize, and give meaning to issues such as organ donation [ 11].
Enumerating the different agents is well beyond the scope of this article, however only a small minority are in common clinical use for detection of bone metastatic disease.
Colonies per well were enumerated, and the mean±s.d. was determined.
The total number of bacteria in each well was enumerated to give effluxed bacteria (EB; effluxed plus intracellular bacteria).
The plates were then incubated for 24 h at 37°C, and the total number of colony-forming units (cfu) per unit area (log10cfu/cm) of microtiter plate well was enumerated.
Bacteria in another set of wells were enumerated without the washing step to give the total bacteria (TB).
On day 2, the intracellular bacteria in one set of wells were enumerated after washing off the gentamicin to give overnight intracellular bacteria (OB).
Cells were stained for microtubule associated protein-2 (MAP-2), a neuronal marker, and the number of neurons remaining on each well was then enumerated using ImageXpressMicro.
Additionally, at each time point, several wells were stained using Trypan Blue (Sigma: St . Louis MO) and live cells were enumerated to determine viability, as previously described [ 30, 31].
Plaques were then enumerated in each well.
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