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Apart from the noradrenergic pathway genes (DBH, DCC, GATA2, GATA3, PHOX2A, PHOX2B, SLC6A2, SLC18A1, and TH) [ 23], several previously reported genes have in this study been verified as differentially expressed genes, i.e. CACNA2D3, DPYSL3, GNB1, POU4F2, RAPGEF6, SLC35E2, and TFAP2B (Table 2 and 3) [ 14, 15, 18, 32- 37].
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Two of these genes, IMP1 and AGTR1, were verified as differentially expressed by qRT-PCR analysis, possibly due to the increased sample number used in each group for this analysis.
All miRNAs selected based on differential expression were verified as markedly differentially expressed, with p-values varying from 10-7 to 10-21 and FC values up to 95 (Table 3).
Selected differentially expressed proteins in cell samples were verified as previously reported [ 23].
None of the genes, which were found differentially amplified by RAP-PCR fingerprint in the laser dissected ileal PP follicles, were verified as significant regulated (P < 0.05) by real-time PCR.
Quality of genotype data was verified as described previously [24].
The significant digits were verified, as suggested.
Specificity was verified as described [ 8].
All samples were verified as Caucasian descendants.
NDN is verified as an epigenetic target.
A significant number of these differentially methylated loci have been verified and these data reported elsewhere[24].
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