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Objective measurement tools, which overcome such considerations, have been validated using controlled laboratory protocols.
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The method was validated using control plants and soil as blank samples spiked with 0.01 μg g−1 and 10 × LOQ 0.1 μg g−1.
Genotyping assays for each polymorphism were validated using control samples of known homozygote wild-type, heterozygote, and homozygote variant genotype generated by direct sequencing.
The assay was validated using control human plasma spiked with known amounts of DMXAA, and found to be linear from 50 n M to 2 m M. Intra- and interassay recovery was between 102 and 104% and CV ranged from 0.2% at 20 μ M to 1.9% at 500 μ M. Free plasma DMXAA concentrations were determined by ultrafiltration.
All primers have been validated using appropriate controls and negative and positive controls for all targets were always included in all PCR runs.
The immunohistochemical analysis was validated using positive controls (tonsil) and negative controls (by omitting the primary antibody).
Labeling specificity was validated using negative controls without primary antibody (Fig. 1C2) or with the peptide antigen (Fig. 1C3).
Gene expression analysis also should be validated using internal controls to represent consistent mRNA expression levels, regardless of bacterial growth conditions [24].
The method to determine positive cells using these antibodies was validated using isotype control antibodies in earlier experiments.
This HPLC method was validated using quality control samples and standard of calibration obtained from spiked blank plasma samples with different concentrations of gemcitabine.
All results were validated using quality control strains: MRSA ATCC 43300, MSSA ATCC 25923 and S. epidermis ATCC 12228 Susceptibilities to common antibiotics were determined by modified Kirby-Bauer disk diffusion methods according to the Clinical Laboratory Standards Institute, formerly NCCLS guidelines [ 11].
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