The PCR restriction fragment length polymorphism (PCR-RFLP) was validated to detect benzimidazole-resistant and benzimidazole-sensitive C. gloeosporioides isolates recovered from avocado, banana, carambola, dragon fruit, grape, guava, jujube, lychee, papaya, passion fruit and wax apple.
Furthermore, the BKPyV qPCR was validated to detect BKPyV genotypes I IV.
These markers have been analytically validated to detect LOH and were selected post-qualification studies using surgically resected EAC specimens microdissected at sites of intestinal metaplasia, dysplasia, and EAC, with histological classification at each site representing consensus of four GI pathologists.
The anti-HECTD3 antibody was validated to specifically detect the HECTD3 protein by IHC (Supplementary Figure S5B).
Single-locus arrays were first validated to detect exonic and intronic CNMs within the DMD locus and the CFTR locus in patients suspected of having mutations in these genes.
The GHQ is a self-administered questionnaire that was designed and validated to detect psychiatric disorders in non-psychiatric settings [ 31, 32], but has been widely used to capture the harms of screening [ 33, 34] given the face validity of questions, which focus on anxiety, depression, hypochondria, and social dysfunction.
In order to maximize detection accuracy (sensitivity and specificity) in commonly impure clinical specimens, the test was optimized and validated to detect genomic alterations with high accuracy 4. Phosphoprotein analysis was also performed on the FFPE samples using a reversed phase protein microarray (RPMA) platform at a CLIA-certified laboratory (Theranostics Health, Inc., Rockville, MD).
An algorithm was implemented and validated to detect the initial and final instants of diaphragm contraction, and the results were compared with the direct measurement of the diaphragmatic muscle length changes made by sonomicrometry [ 8].
In summary, a series of diagnostic assays were developed and validated to detect MusPV infection in skin tumors and serological response in laboratory mice.
