This method has been validated by counting the number of isl1-expressing cells at 24 hpf after a visible WISH or after a fluorescent WISH analyzed by confocal microscopy; very similar results were found (a mean of 49 cells counted by the first method and of 47 by the second) (see Figure 7 in [ 50]).
The accuracy of the counting results has been validated using expert counted cell numbers as the gold standard.
Similarly, fragility fractures recorded as non traumatic may not have been validated so any count may have included some trauma fractures.
The ActivPal has been successfully used in studies of office workers and adults, 16 17 and has been validated for step count, cadence, time spent sitting, standing, walking and for identifying postural transition.
In this case, they investigated a particular form of hubris — narcissism — by using a simple and unobtrusive gauge that has been validated by psychologists: just count the number of times a person uses the first-person pronoun in communication.
The grading scale has previously been validated by comparisons to axon counts [ 42, 43].
Actical accelerometer count thresholds have subsequently been validated using calorimetry to classify time spent sedentary, and in light, moderate, and vigorous intensity physical activity in children [ 36].
19 Cough frequency measured using the Leicester Cough Monitor has been validated against manual cough counts and subjective cough outcome measures, including cough visual analogue scales and the Leicester Cough Questionnaire.
If claims of interpretation and use from an assessment cannot be validated, then "they count against the test developer or user" [ 11].
In this work, the percentage of the damaged area of each damaged building is also calculated by a newly introduced texture-wavelet analysis on roof-tops, and the results are validated by counting the damage pixels manually.
