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While daily home collection of mucosal samples for herpesvirus culture and PCR has been validated in multiple studies in resource-rich settings, [11], [12], [20], [21], [22], [23], to our knowledge this is the first study to perform intensive mucosal sampling in a resource-limited setting.
The Version III (RUGs III) system has been validated in multiple settings [ 72- 74].
The accuracy of coding of the comorbidities has been validated in multiple publications [ 22, 23].
With this in mind, we investigated nine signatures that have received great interest and been validated in multiple studies.
The obtained CO and preload and afterload variables have been validated in multiple studies [ 12, 39- 42].
The Framingham heart predictive tool is the most widely used instrument in clinical practice to help predict heart disease and has been validated in multiple populations.
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Significance of NF-kB targeting by bortezomib was validated in multiple myeloma cells where genes that belong to NF-kB pathwerewere significantly overexpressed in samples associated with response to bortezomib [3], [4].
21 Several rules may predict the same outcome and, in parallel, a single rule may be validated in multiple populations and settings.
Anti-TOX3 antibody, henceforth referred to as AJ-33, was purified by protein A affinity chromatography and was validated in multiple assays reported here.
Importantly, editing sites located in non-repetitive editing boxes were validated in multiple human cell lines using conventional PCR and Sanger sequencing and were proven to be catalyzed by ADAR1.
The putative therapeutic potential of Uncl-Sema3E was validated in multiple orthotopic or spontaneous tumour models in vivo, where either local or systemic delivery of Uncl-Sema3E-reduced angiogenesis, growth and metastasis, even in the case of tumours refractory to treatment with a soluble vascular endothelial growth factor trap.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com