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The primer pairs have been validated by gel electrophoresis, DNA sequence analysis and thermal denaturation profile.
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Moreover, EWS-FLI1 direct interaction with these repeated elements was validated by gel shift assays [33].
α1A-AR, cav-1, cytokeratin 14 and 18 mRNA expressions were validated by gel density analysis by using GAPDH mRNA as internal control.
All primers were designed to span an intronic sequence and were validated by gel analysis.
Polymerase chain reaction (PCR) findings were validated by gel electrophoresis, and PCR products were purified with a QIAquick PCR purification kit according to the protocol described by the manufacturer (Qiagen).
After all the gaps were filled with appropriate qualities, assembling of the genome DNA was validated by gel electrophoresis of its genomic DNA and re-mapping of the short DNA reads, resulted in no inconsistency in the assembling.
But now they have been validated by his own board.
You've been validated by your teachers and peers.
PCR products were validated by agarose gel electrophoresis and melting curve analysis.
A total of 14 genes with relevant expression profiles from the Oligo array data were chosen to be validated by RNA gel blot analysis.
The PCR products were validated by agarose gel analysis which demonstrated bands at ~854 bp for both templates, accordingly to the size of the sequence.
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