Sentence examples for been validated by conventional from inspiring English sources

The results have been validated by conventional immunohistochemistry and Western blotting [36].

The predictive capabilities of the optimal MLP models are validated by conventional recommended correlation and a large number of experimental data which were collected from various literatures.

The combination of information taken from the different techniques allows the measurements obtained to be validated by conventional and modern approaches and the accuracy of each to be enhanced.

Mutations identified by TILLING were validated by conventional sequencing as previously described [ 37].

The SNP in inhA was validated by conventional Sanger sequencing using an ABI3130XL genetic analyser (Applied Biosystems).

Putative somatic SNVs were validated by conventional Sanger-based sequencing analysis of PCR products obtained from the tumor WGA DNA.

All the Gateway entry clones obtained were validated by conventional Sanger DNA sequencing before cloning into Gateway destination vectors.

All candidate pathogenic variants not previously identified were validated by conventional PCR amplification and Sanger sequencing and were not detected in 284 control chromosomes.

The quality of purified DNA from plasma samples was validated by conventional PCR amplification of the humanβ-globin gene using gene-specific primers (βF: 5'-AGGAGTGGTGGCTCATGTCT-3' andβR: 5'-CTCAAGGGATCCTCCCATTT-3').

Thus, global gene expression technologies such as microarrays can be used to identify a specific gene marker, or a suite of markers, that can then be validated by conventional methods such as Northern blot analysis, in situ hybridization, and quantitative polymerase chain reaction.

Currently, real-time PCR (RT PCR is increasingly being used for both research and clinical applications. For BC in particular, the detection of HER2 gene amplification has been validated by comparison with conventional methods, such as FISH (Lamy et al, 2006).