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We further investigated whether these angiogenic factors are co-regulated by these miRNAs, which have been validated as regulators of VEGF in CNE cells.
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Moreover, miR-203 has been validated as a regulator of Bmi-1, which is a member of the Polycomb repressive complex 1 (PRC1) family of proteins and is also involved in epigenetic modifications [ 47].
Interestingly, the miR-17-92 miR-17-92 miR-17-92lidated as an oncluster regulator and at thassame time as a tumour suppressive regulator.
Two specific chloride channels ClC-2 (chloride channel protein 2) and CFTR (cystic fibrosis transmembrane conductance regulator) have been validated as targets for the treatment of constipation.
PTEN expression is frequently decreased in a wide spectrum of human cancers with several miRNAs being validated as PTEN regulators [ 37].
Using a luciferase-based assay, miR-26a, miR-101, and miR-98 were validated as bona fide regulators of EZH2 expression.
All primers were validated as described [55].
For example, Cnot4 has previously been shown to act as a pathway regulator in S2 cells and has been validated using cell-based and in vivo assays [ 26].
"His vision has been validated".
"Everything has been validated.
We've been validated.
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