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The final pH of the resultant suspension was pH > 7. Bioassay techniques in the mouse have been validated and optimized as described previously (Wegesser and Last 2008).
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All assays were validated and optimized at the Core Genotyping Facility laboratory.
PCR conditions were validated and optimized by using R. typhi- and R. felis-positive controls.
Such models must therefore be validated and optimized in each setting.
During the multicenter study the cut-off was validated and optimized by receiver operating characteristic analysis (see later).
As a result, expression data for genes whose sequence information is available can be validated and optimized while still providing an insight into overall expression trends for all the genes represented in the GeneChip.
Our data collection relies on tools that have been validated and extensively used in research, and we have adopted strategies to optimize the response rate.
Based on test results, the numerical model is validated and the shell design is optimized by virtual testing.
Plastic adherence of PBMC obtained from leukapheresis for generation of monocyte-derived DC is the most widely used technique because of easy handling and cost-effectiveness and has been extensively validated and optimized for closed cell culture systems according to GMP guidelines for patient application [ 30, 31].
The analytical procedures were validated to optimize sensitivity and selectivity.
This paper addresses NG leakage surveillance through a robust cooperative/synergistic scheme between biosensors and conventional detector systems; the network is validated in situ and optimized in order to provide reliable information at the required granularity level.
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