Subconfluent cultures (80 90% confluence) were utilized in experiments.
BMMC were utilized in experiments after 4 weeks in culture and showed 99% mast cells when stained with toluidine blue.
Cells were trypsinized with 0.05% Trypsin-EDTA for 10 minutes, centrifuged at 900 rpm for 3 minutes and counted using the Trypan Blue assay on a Countess® automated cell counter (Life Technologies); only passages identified as greater than or equal to 95% viable were utilized in experiments.
It has been utilized in several experiments concerning intrinsic motivation and self-regulation.
D'orenone has been utilized in this experiment because it increases PIN2 protein abundance without affecting PIN2 transcripts, with the consequence that the PIN2 expression domain enlarges and shifts basipetally, resulting in more active auxin transport.
The tissue-like mechanical properties of these hydrogels have also been utilized in early OCE experiments [ 4].
The probe 7 showed excellent biocompatibility and has been utilized in cellular imaging experiment to detect Fe3+ in MCF-7 live cells.
Although RAGE KO mice have been utilized in biochemical and physiological experiments to address the roles of RAGE in progression of various pathological conditions, consequences of lacking RAGE in normal condition have hardly been addressed.
For these reasons, the various acetyltransferase inhibitors have been utilized in a variety of experiments aimed at measuring their biological effects.
Material I (50%DM) was used in experiments without washing between two steps while material II (38% DM) was utilized in the experiments with washing the solids.
