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Paramagnetic contrast agents have also been utilised for cell tracking studies to monitor T-cell homing in vivo after loading T cells with superparamagnetic iron oxide (CLIO) nanoparticles conjugated with a peptide sequence from the transactivator protein (Tat) of HIV-1.
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These have been utilised for designing the AR-HRC.
The surface fabrication techniques presented here can be utilised for the preparation of cell-based biosensors as well as tissue engineering constructs.
A newly designed coaxial-tube electrochemical ESR cell is utilised for the detection of paramagnetic species electrogenerated in situ at variable temperatures.
Hela cells, which are a homologous cell line, was utilised for the transactivation studies.
MCF-7 human breast cancer cells were utilised for the anti-proliferation study.
Following 12 h of infection, in the presence of 8 μg ml−1 polybrene, and 48 h of gene expression, infected cells were utilised for experiments, without further selection in G418.
Two or more cell lines were utilised for each organ site to obtain corroborative results.
We conducted a feasibility study to examine whether small numbers of cancer cells could be utilised for analysis of the EGFR gene status using the loop-hybrid mobility shift assay, which is a modified heteroduplex technique.
Despite the fact that many studies have shown that eEPCs can promote therapeutic neovascularisation in ischaemic tissues [ 29], our data suggests that these cells should be utilised for therapy with caution and consideration of the underlying pathology they will treat.
We investigated whether bombesin/gastrin releasing peptide receptors found in glioblastoma cell lines can be utilised for targeting of a cytotoxic bombesin analogue, AN-215 consisting of a potent derivative of doxorubicin, 2-pyrrolino-doxorubicin (AN-201) linked to a bombesin-like peptide carrier.
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