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In mice, several models have been used for transcriptome profiling.
The Illumina platform has been used for transcriptome analysis in several plant species [ 27– 327.
This platform has been used for transcriptome sequencing of pine [ 30], oats [ 31], Aegilops [ 32] and buckwheat [ 33].
Exactly the same two RNA samples (one from a tame and one from an aggressive fox) which had been used for transcriptome sequencing were used for RT-qPCR validation experiments.
RNASeq has previously been used for transcriptome characterization of non-model species, including butterfly [ 20], silkworm [ 21], garter snake [ 22], coral [ 23], pearl oyster [ 24] and several fish species [ 25- 30], including rainbow trout [ 31].
In contrast, in bacteria, this approach has only recently been used for transcriptome analysis, although it has the potential to increase our insight into transcriptional and post-transcriptional events in microorganisms dramatically [ 20- 22].
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Liver RNA from 7 healthy cows was used for transcriptome profiling using a bovine microarray.
Affymetrix microarrays were used for transcriptome profiling between parental and the highly invasive subline.
Cufflinks (version 2.2.1) was used for transcriptome assembling, and the Cuffmerge script from Cufflinks was used to merge the transcript files into a single transcriptome annotation.
Next, RNA-seq reads were mapped to the human transcriptome using Tophat2 program version 2.1.159; the genes.gtf file in the Illumina iGenome hg19 was used for transcriptome annotation.
The RNA was collected from the infective extracellular form, the elementary bodies (EBs) and vegetative intracellular form, reticulate bodies (RBs) and was used for transcriptome sequencing.
More suggestions(15)
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