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Traditionally, small nuclear- or nucleolar RNAs like RNU6B have been used for normalization in miRNA experiments.
Only those that mapped uniquely to the mouse genome were used for further analysis, and their number has been used for normalization to compare signal difference between libraries.
GAPDH and ACTB have been used for normalization in more than 90% of previous reports [ 41], often without proper validation of their stabilities.
Sarcomeric alpha-actinin: Ensembl gene Actn2 ENSmouse0000052374 (mouse) and ENSG00000077522 (human) and beta-actin: Ensembl gene Actb, ENSG00000075624 (human) have been used for normalization.
If the entire complement of genes on the array had been used for normalization, rather than using a set of 1000 genes with sequence conservation, then there would have been an even greater increase in the false positive rate in D. simulans (39%) and D. yakuba (67%) relative to the platform species, D. melanogaster (12%).
RACK1, which has previously been used for normalization of light-dependent gene expression in the closely related algae Chlamydomonas reinhardtii [ 10, 11] and shows constitutive expression in both reproductive and somatic cells, was used for normalization of cell type specific light-mediated gene expression data.
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Commercial normal breast cDNA was used for normalization.
GAPDH was used for normalization.
GAPDH was used for normalization of expression.
A pool of all the samples was used for normalization.
GAPDH was used for normalization of RT-qPCR data.
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been chosen for normalization
been used for anything
been used for hashing
been used for batch
been used for co-segmentation
been proposed for normalization
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com