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A compatible set of 96 barcode sequences that have been used for multiplex sequencing is provided as supporting information (Table S1).
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A biotinylated consensus primer, GP6+, and 15 high-risk HPV type-specific primers are used for multiplex PCR.
PrimerPlex 2.0 (Premier Biosoft International) was used for multiplex PCR signature design.
Two primer sets (PCR1: bbp, cna, ebpS and eno; PCR2: fnbA, fnbB, fib, clfA and clfB) were used for multiplex PCR.
In a recent study by Ingvarsson et al, 2007, sandwich antibody microarrays were used for multiplex analysis of eight complement proteins (including C3) in unfractioned serum and plasma [23], showing promising results for protein profiling in complex samples.
Both the EpiTect Methylight and QuantiTect Multiplex Mix buffer systems could be used for multiplex qMSP.
Markers that yield positive signals in one plate can be used for multiplex PCR.
Two primers, forward (F) and reverse (R), were used for multiplex PCR amplification.
Sequenom MassARRAY® software (Sequenom) was used for multiplex SNP analysis design.
This approach appears feasible for individual miRNA marker quantification but cannot be used for multiplex miRNA evaluation.
The specimens without perfusion were used for multiplex polymerase chain reaction (PCR), in order to detect possible infections.
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