Sentence examples for been transduced with a from inspiring English sources

Nine years ago, we transplanted two dogs (E900 and E958) with autologous marrow CD34+ cells that had been transduced with a gammaretrovirus vector encoding a conditionally activatable derivative of the thrombopoietin receptor.

As a source of LIF, we used conditioned medium from Plat-E cell cultures that had been transduced with a LIF-expressing vector.

Moreover, the percentage of Ngn2-hENPs grafted animals that reached a grid score ≥3 was significantly higher when cells had been transduced with a non-integrating vector than with an integrating vector (Figure 1E).

For example, when NOD-SCID mice were transplanted with human neurospheres that had been transduced with a lentiviral vector expressing green fluorescent protein (GFP), more than 98% of 200 transplanted animals had human cell engraftment.

We selected three independent cell clones (Sh-Ncad1.1, Sh-Ncad1.2 and Sh-Ncad2) that displayed reduced N-cadherin expression in comparison to the control cell line that had been transduced with a scrambled shRNA (Sh-Scr).

To investigate whether human Δ133p53 α also stabilizes FLp53, we used A549 lung cancer cells that had been transduced with a retrovirus expressing Δ133p53 α. Results show that Δ133p53 α stabilizes FLp53, however, like transduced Δ122p53, transduced Δ133p53 α caused a decline in p21CIP1 levels, although this substantially recovered by 24 h after treatment.

Recombinant adenovirus expressing hepatitis B surface antigen (HBsAg) (Ad-S) was constructed, and bone marrow-derived DCs were transduced with Ad-S or pulsed with HBsAg protein.

Transfected cells were transduced with Ad-β-gal 48h later.

To determine the potential for UCP-2 to regulate insulin secretion in human islets, they were transduced with Ad-dnUCP-2 or Ad-GFP and perifused (Fig. 2C).

Three days later, islets were transduced with Ad-GFP or Ad-dn-UCP-2 at 3×106 pfu/islet as described above.

Primary hepatocytes from control mice were transduced with Ad-control or Ad-DN Atf2 and treated with tunicamycin.