We first summarize the key regulatory factors involved in controlling the self-renewal and pluripotency of ES cells, which have been thoroughly evaluated using various systems biology tools.
As the variation among bisulfite modifications previously has been thoroughly evaluated using various PCR-based methods for single locus methylation detection [ 27, 28], we bisulfite modified a subset of the samples a second time, and tested for CDKN2A methylation using MS-HRM.
Finally, the uncertainty range of reservoir heterogeneity is thoroughly evaluated using a large number of geological realizations with significant differences on porosity and permeability distributions.
This regression model was thoroughly evaluated using highly active MTB inhibitors absent in the training dataset, as well as inhibitors of the validated MTB drug target InhA.
Parameters affecting the performance of the microextraction process were thoroughly evaluated using uni- and multivariate optimization strategies, based on the use of experimental factorial designs.
Mixtures of the stabilized soils were thoroughly evaluated using compaction, California Bearing Ratio (CBR), unconfined compressive strength (USC) and durability tests.
There have been efforts to alleviate loneliness, especially as manifested in social isolation, but only a minority of interventions have been thoroughly evaluated with the use of sound methodologies, and social and emotional loneliness have rarely been distinguished (Andersson, 1998; Cattan, White, Bond, & Learmouth, 2005; Findlay, 2003; O'Luanaigh & Lawlor, 2008).
The identification of PI animals (for subsequent elimination) is an essential element in any BVDV control programme, and depends on accurate diagnostic tests, i.e. tests with high sensitivity and specificity that have been thoroughly evaluated for routine diagnostic use.
The non-invasive methods used have all been thoroughly evaluated, and reproducibility and repeatability have been reported.
Chronic disease associations with short duration, low dose use have not been thoroughly evaluated.
Many agents have been used in the past but none of them have been thoroughly evaluated.
