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Even though such a result in a clinical situation needs further confirmation, the diagnosis might have been missed if the qPCR assay had not been tested in duplicate.
Eleven gDNA Salmonella samples from pure culture at 10 copies per assay have been tested in duplicate with the three detection systems.
Of these, 57 (30%) had been tested in duplicate, of these 57 duplicates, 3460%0%) supported the original copy number call and 23 supported the alternative copy number call (Additional 6: Figure S4).
Similar(57)
The samples were tested in duplicate.
Each serum sample was tested in duplicate.
Every combination (kinetic ∗ amplitude) was tested in duplicate.
At AnaMar AB, all the samples were tested in duplicate for COMP and CILP-2.
These independent variables were tested in duplicate at three levels, while dry biomass productivity was used as the response.
All peptides were tested in duplicate.
All patient samples were tested in duplicate.
All experimental samples were tested in duplicate.
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