The second program is an integral part of the first program (ref. to Response 4 to Reviewer 2), and therefore they are tested in complex.
To our knowledge, the influence of Aβ on MTT reduction has never been tested in more complex tissue.
Although many in vitro findings on Aβ toxicity and competing, protective agents are based on the MTT assay [11] [14], the influence of Aβ on MTT reduction has never been tested in more complex models than single cell cultures.
CDM rules have not been tested in those complex communities that harbour tremendous biosphere carbon management potential.
More recently, the epigenetic approach has been tested in a more complex manner using a combination of fetus-specific genetic (ZFY on the Y chromosome) and epigenetic markers (holocarboxylase synthetase, HLCS, on chromosome 21) [ 30].
While multi-agent based Cloud management architectures or frameworks were proposed before mOSAIC (like in the proposal exposed in [25]), we consider that mOSAIC's Cloud Agency is the most complete implementation of the concepts that has been tested in the context of complex scientific and commercial applications.
These three methods (Pareto/LCA, DoE/LCA and CSP/LCA) have been tested in an ecodesign project for a complex system: a hybrid passenger ferry.
The antitumor capacity of the complex has been tested in vitro against human cancer cell lines, cervical carcinoma (HeLa) and colorectal adenocarcinoma (Caco-2), by metabolic tests, using 3- 4,5-dimethylthiazol-2-yl -2,5-diphenyl-tetrazolium bromide as reagent.
However, delivery of therapeutic genes via a liposome-mediated, MC DNA complex has never been tested in vascular-rich hepatocellular carcinoma (HCC).
The resulting complex is under test (in Systems and Software Engineering Laboratory at Tallinn Technical University), separate tools have been tested in two European Union research projects (CP-94 no. 1577 LIMITS, and ESPRIT 22154 BRIDGE).
