10 µg of cell lysate was tested for reverse transcriptase activity using a C-type-RT activity assay (Cavidi Tech) following the manufacturers' instructions.
The performances of the developed models have been tested for both forward and reverse mappings with the help of some test cases collected through the real experiments.
This concept of hybrid fixation is now commonplace in fracture management using locked plates, but prior to this study had not been tested for applicability in the reverse shoulder replacement glenoid component.
Many different stationary phases have been tested for this purpose, including reversed-phase [ 10, 12, 13, 17- 21], normal phase [ 22- 24], and ion exchange [ 25- 36].
Samples were tested for HBsAg by reverse passive hemagglutination assay (Wellcotest®, Murex Diagnostics, Dartford, UK) and later by Determine™ HBsAg (Abbott Laboratories, Illinois, USA), an immunochromatographic assay.
All stool specimens were tested for NoV by reverse transcription polymerase chain reaction, as previously described (5 ).
Samples were also injected into MRC5 cell monolayers for virus isolation, and they were tested for HMPV by reverse transcription PCR.
Nasal and throat swabs were tested for influenza with reverse transcription PCR (RT-PCR) by using primers and probes provided by the Centers for Disease Control and Prevention (Atlanta, GA, USA) (8 ).
Anti-HBc positive samples were tested for HBsAg by reverse passive hemagglutination assay (Wellcotest®; Murex Diagnostics, London, UK) and/or by the Determine™ HBsAg immunochromatographic assay (Abbott Laboratories, Abbott Park, IL, USA).
Serum samples were tested for RNA by reverse transcription-nested PCR by using primers designed within open reading frame (ORF) 1 and ORF2 (13, 13 ).
