Conditioned media and cell lysates were tested for expression of the A-domain and individual cell lines that expressed the A-domain to the highest extent were then used in subsequent experiments.
WT and ΔCS mice were tested for expression of mFasL and sFasL.
To pinpoint modules specifically associated with stemness, all-for-all modules were tested for expression exclusive to stem cells.
EDCs were tested for expression of GATA-4, MyoD, ANF and NKx2.5, using primers from Invitrogen, UK (See table S1).
All constructs were tested for expression and their ability to target the reporter construct determined in polytene chromosomes (Figure S5).
amiRNA117/105 plants were tested for expression levels of both target genes in inflorescences by qRT-PCR analysis.
Next, these cells were tested for expression of the mammary gland cell-lineage specific markers CK5, CK14, WAP and β-casein.
The resulting Th1, Th2, Th17 cells were tested for expression of IFNγ, IL-4, IL-17 cytokines by flow cytometry and expression of Tbet, GATA3, RORC, and FOXP3 transcripts by quantitative real-time PCR (qt-RT-PCR).
The constructs were tested for expression in LMC500 (MC4100 lysA), a well characterized laboratory strain [20], [21], MC1061 ΔOmpA [5], an OmpA knockout strain, and in its parental strain MC1061 [22].
Transiently transfected MKK mutants were tested for expression, resistance to LT cleavage and their ability to relay exogenous signals to specific downstream MAPKs in LT-treated cells (Figure S5A).
