Four classical nonorthogonal JDC methods without nonnegativity constraints including ACDC [23], FFDIAG [25], LUJ1D [26], and QRJ1D [26] and one nonnegative JDC method ACDC LU + [41] are tested as reference methods.
Ribosomal protein 13 and 19 (rpl19, rPeptidylprolyllprolyl isomerase Aa (ppiaa), elongation factor 1 alpha, Glyceraldehyde-3-phosphate dehydrogenase (gapdh) and hypoxanthine phosphoribosyltransferase 1 (hprt1) were tested as reference genes.
We selected nine genes to be tested as reference transcripts (ACT, CYP, EF1α, GAPDH, RAN2, RPS13, SAND and UBQ) based on previous descriptions (see below) (Table 1).
One cold-adapted A (H1N1) and one cold-adapted B virus strain was tested, as well as the reference strain A/PR/8/34 (H1N1).
The CR20L samples were tested as-received with spectral referencing with respect to nitromethane.
The samples were cut in small pieces and tested in duplicate; (v) control samples: BC and CV fibers without the addition of Cu were tested as blank reference, while as internal reference of the method the bacteria growth was tested on flasks only containing inoculated Broth media.
The samples were cut in small pieces and tested in duplicate; control samples: BC and CV fibers without the addition of Cu were tested as blank reference, while as internal reference of the method the bacteria growth was tested on flasks only containing inoculated Broth media.
