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A first step in this direction has been taken for microarray data by Ahmed and Xing (2009).
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Since the regulatory roles of C. acetobutylicum CcpA in fermenting d-glucose and d-xylose mixture (simulating lignocellulosic hydrolysates components) are of great interest, these two mono sugars were used as the carbon sources in our fermentation experiments, from which samples were taken for microarray assay.
QPCR was performed using an RNA extract from the same five animals that had been taken for enzymic and microarray analysis.
Slides from FFPE tissue blocks were evaluated by two genitourinary pathologists (D. M. B. and J. A. B .. Tumor-bearing areas were identified, and 0.6-mm cores were taken for tissue microarray (TMA) construction and DNA extraction.
Of the 60 cases where tissue was available, 2 cases had insufficient tumour tissue for DNA extraction or for a core to be taken for assembly of a tissue microarray (TMA) and a further single case had an extremely low DNA yield and insufficient material for tissue microarray.
Upon rigorous examination of the resulting diagnostic plots, 116 microarrays of the supreme quality were taken for the further analysis.
Pretreatment biopsies (incisional) were taken for histopathology, prognostic marker evaluation (hormone receptor and HER2), and DNA microarray analysis.
CEL files generated by the Affymetrix GeneChip Operating System were taken forward for microarray data analysis.
Where the target mRNA was knocked-down 30% or more on average in the presence of IFNγ the samples were taken forward for microarray analysis.
In [ 17], C. elegans gene expression data for were taken from 553 microarray experiments over a variety of experimental conditions, including different growth conditions, developmental stages and mutants.
Isolated RNA was taken forward for expression microarray analysis using the Affymetrix Genechip HG-U133 Plus 2.0 array (GeneService Ltd .. Data are available at ArrayExpress (http://www.ebi.ac.uk/microarray-as/aer/) under the accession number E-MEXP-1522 E-MEXP-1522 E-MEXP-1522
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