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The method for identifying TSGD paralogs that have been systematically retained as pairs in one superorder but as a single copy in the other is schematically illustrated in supplementary figure S1, Supplementary Material online.
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This study has shown that some TSGD paralogs have been systematically retained in Acanthopterygii but Ostariophysi superorders of teleosts (1.3% and 2.6% of total gene content, respectively).
Similarly, 1.3% (418) of total gene number represented cases where TSGD paralogs pairs were systematically retained in the Acanthopterygian but conserved as a single copy in Ostariophysi genomes.
Using a similar rational, we found that orthologs of 113 genes representing 226 TSGD paralogs (1.3% of total gene content) were systematically retained in all Acanthopterygii genomes tested (Oreochromis, Tetraodon, Oryzias, and Gasterosteus) but as a single copy in the two Ostariophysi genomes available (Danio and Astyanax).
Interestingly, 119 SCPCH out of the 246 of FUNYBASE were systematically retained among 30 of the 40 searches.
To identify those duplicates that were systematically retained in Ostariophysi superorder, D. rerio paralogs with a single best hit against the same G. aculeatus (Gasterosteiformes) ortholog were retrieved.
Women have been systematically raped as a method of intimidation.
The public servants' ethos, their attachment to the civic realm, has been systematically trashed as mere unionised self-interest.
These NRs have been systematically defined as NR2E1 through NR2E5.
DSTRKT has been systematically deleting the posts but as fast as they do, they are re-uploaded.
Exploiting or even seriously assessing that potential has been systematically suppressed for decades as a fatal competitor to nuclear power.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com